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Baculovirus Expression Systems

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Home > Protein Analysis > Protein Expression > Baculovirus Expression Systems
Protein Expression
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Baculovirus Expression Sysrems

TaKaRa Bio

Tools for generate large amounts of a recombinant protein

Baculovirus Expression Systems
TaKaRa Bio
  • Uses the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) to produce target proteins in insect cells.
  • The target gene is inserted into a shuttle vector, which is cotransfected into insect host cells with the linearized BacPAK6 Viral DNA.
  • The specially designed BacPAK6 Viral DNA forces recombination between the virus and transfer vector, resulting in high recombination efficiency.
  • Following recombination, a few viral plaques are picked and purified, and the recombinant phenotype is verified.
  • The newly isolated recombinant virus can then be amplified and used to infect insect cell cultures to produce large amounts of the desired protein.

 

 

Documents and resources

For more information visit producer's website. 

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