cDNA Synthesis

TaKaRa Bio

1) PrimeScript RTase

• MMLV RNase H minus RTase with strong extension activity
• Capable of extending up to 12 kb templates with minimal background
• cDNA synthesis and extension through complex RNA structure at 42 °C
• High specificity: reduced mispriming increases reverse transcription efficiency
• High accuracy reverse transcription

 

 

2) RNA to cDNA EcoDry Premix

• Lyophilized premix including RTase, dNTP, buffer and primers: just add RNA template in water
• UltraPure SMART MMLV RTase
• Synthesis of high-quality first-strand cDNA of up to 11.7 kb, from virtually any transcript
• Eco-friendly format: storage and shipping at room temperature
• Supplied in 8 PCR tube strips: unprecedented flexibility and ease-of-use

 

 

3) PrimeScript 1st Strand cDNA Synthesis Kit

• Excellent elongation: able to synthesize long cDNAs (up to 12 kb) with good yield
• Oligo dT and random primers included: use of polyA and total RNA templates
• Robust and efficient: carries out reverse transcription at standard RT temperature (42 °C), even when RNA template contains high-order structures

 

 

4) PrimeScript II 1st Strand cDNA Synthesis Kit

• Improved specificity with accessory protein: icreases RT efficiency by avoiding mispriming
• Excellent elongation: able to synthesize full length cDNAs with good yield
• Oligo dT and random primers included: use of polyA and total RNA templates
• Robust and efficient: carries out reverse transcription at standard RT temperature (42 °C), even when RNA template contains high-order structures

 

 

5) PrimeScript Double Strand cDNA Synthesis Kit

• Includes the high efficiency enzyme PrimeScript Reverse transcriptase
• Synthesizes first strand cDNA from purified polyA+ RNA
• The resulting double-stranded cDNA can be cloned into an appropriate vector to generate a cDNA library
• Contains a positive control RNA: double-stranded cDNA synthesized from the positive control RNA tempalte and cloned results in a plasmid that confers tetracycline resistant

 

 

Documents and resources

For more information visit producer's website.