CRISPR/Cas9 allows site-specific genomic targeting. The CRISPR/Cas9 system has been harnessed to create a simple, RNA-programmable method to mediate genome editing in mammalian cells, and can be used to generate gene knockouts (via insertion/deletion) or knockins (via HDR). Takara Bio offers solution for all steps of the workflow from sgRNA synhesis and delivery options up to mutation detection.
1. IN VITRO TRANSCRIPTION
Guide-it sgRNA In Vitro Transcription Kit
- All reagents necessary for sgRNA synthesis
Guide-it™ IVT RNA Clean-Up Kit
- Kit for purification of sgRNA after in vitro transcription
Guide-it™ Complete sgRNA Screening System
- Complete solution for sgRNA production, cleanup and screening
- PCR enzyme for genomic target amplification and Cas9 included in the kit
Guide-it™ sgRNA Screening Kit
- Kit for selecting the best guide sequences
- Cas9 included
Guide-it Long ssDNA Production System
- ssDNA donor templates production (500–5,000 nt)
- Gene knockin applications
- Simple and fast protocol
2. DELIVERY SYSTEMS
Gesicle delivery products
- Kits for production of gesicles with target-specific CRISPR/Cas9
- Gesicles: cell-derived nanovesicles used for cargo delivery into cells
- Have to be used together with pGuide-it-sgRNA1 Vector
Viral delivery systems
- Ideal for genome editing in hard-to-transfect mammalian cells
- AAV delivery of Cas9 precludes genomic integration and persistent expression of Cas9, reducing off-target effects
- Lenti-X Tet-On 3G CRISPR/Cas9 System incorporates the Tet-On 3G transactivator protein, which enables users to induce Cas9 expression via the addition of doxycycline
- Lenti-X CRISPR/Cas9 System involves the constitutive expression of Cas9 in target cells
Xfect™ RNA Transfection Reagent
- Suitable to work with messenger RNA (mRNA), single guide RNA (sgRNA), microRNA (miRNA), and siRNA
- Very low cytotoxicity
3. DETECTION AND SCREENING KITS
Guide-it Mutation Detection Kit
- Complete kit for identification of insertions and deletions in mammalian cells
- Amplification directly from the cells
Guide-it Cas9 Antibodies
- Polyclonal antibody for recombinant Cas9 recognition
- Can detect less than 1 ng of Cas9
Guide-it Indel Identification Kit
- Kit for identification of indels caused by CRISPR/Cas9 and other gene editing methods
- Very fast protocols work directly from cells
Guide-it SNP Screening Kit
- No need for sequencing
- Whole protocol done in 4 hours
- Can detect any nucleotide substitution
Guide-it Knockin Screening Kit
- Detection of single-nucleotide substitutions to longer insertions
Documents and resources
For more information visit producer’s website.
