High Fidelity PCR polymerases

TaKaRa Bio

PrimeSTAR

• Provides high fidelity and accuracy during amplification of large products
• Extremely low error rate
• High tolerance to varying reaction conditions

 

	PrimeSTAR Max	PrimeSTAR HS	PrimeSTAR HS (GC Buffer)
Applications	HTP studies Cloning and expression Structure/function studies	cDNA libraries cDNA cloning Site-directed mutagenesis Sequencing	High GC content PCR cDNA libraries cDNA cloning for protein expression
Features	9 errors/ 230,129bp Fastest extension rate of any available enzyme Hot start	12 errors/ 250,000bp Targets up to 8.5 kb Short reaction time Hot Start Short annealing times	25 errors/ 304,000bp Targets up to 5 kb Hot start Specialised buffer GC Rich
Additional coments	Suitable for rxn’s with ex-cessive nucleic acid (normally inhibits tem-plate amplification)	High tolerance to varying reaction conditions Premix available	For templates with 75% or greater GC content

 

PrimeSTAR GXL DNA Polymerase

• Modified PrimeSTAR polymerase combined with elongation factor
• Unrivaled processivity with high accuracy
• Longest amplification: up to 30 kb human gDNA or 13.5 kb with cDNA
• Robust enzyme buffer combination
• Excellent performance on GC-rich or AT-rich templates and targets containig repeats
• Can be used with samples containing excess nucleic acids
• Tolerates a wide range of template quantity which inhibit other high-fidelity DNA polymerases
• Antibody-mediated hot-start formulation for specific amplifucation

 

Advantage HD DNA Polymerase Mix

• Provides superb accuracy and efficiency
• Produces results fast due to the enzyme’s high priming efficiency
• Generates error-free PCR products from targets of any complexity up to 8.5 kb
• Provides the exact amplification product, maximum specificity results in minimal background
• Features: proofreading function, only 12 errors per 250,000bp, shorter annealing time
• For critical applications such as gene cloning and amplification of cDNAs for library construction, In-Fusion cloning etc.
• Novel PCR enzyme (not same as Advantage 2)

 

CloneAmp HiFi PCR Premix

• Provides exceptionally accurate and efficient DNA amplification
• Quick priming and extension (5 sec/kb) enable fast PCR reactions
• Hot start antibody prevents nonspecific amplification
• Works with long (up to 10 kb) and GC-rich templates
• Produces results fast due to the enzyme’s high priming efficiency

 

Documents and resources

For more information visit producer's website.