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His-Tagged Protein Purification

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Home > Protein Analysis > Protein Purification > His-Tagged Protein Purification
Protein Purification
  • Antibody Purification
  • GST-Tagged Protein Purification
  • Myc-Tagged Protein Purification
  • His-Tagged Protein Purification
  • DYKDDDDK-Tagged Protein Purification
  • Phospho and Glycoproteins Enrichment
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His-Tagged Protein Purification

TaKaRa Bio

Revolutionary solution for recombinant protein purification 

His-Tagged Protein Purification
TaKaRa Bio

For your research you can choos from wade range of available kits

1) Capturem Kits

The Capturem His-Tagged Purification Miniprep Kit

  • The Next Generation of His-Tagged Protein Purification
  • Revolutionary solution for recombinant protein purification
  • This complete system effortlessly outperforms conventional methods
  • Spin columns with high-capacity membranes instead of resin
  • Elute concentrated protein: up to 0.3–1 mg/ml
  • Speed: 5 min at room temperature instead of 1–2 hour in the cold room
  • Flexibility: purify from mammalian or bacterial cells

 

Protein purification miniprep workflow 

 

Capturem Maxiprep Kits

  • Effortlessly outperforms conventional methods
  • Convenient, powerful and efficient research tool
  • Isolate ~ 2.5 mg of your his-tagged protein with a purification system 
  • High-capacity: columns contain novel nylon membranes instead of resin
  • High-yield: elute concentrated protein (up to 4.5 mg/ml)
  • Fast and convenient: work for 15 min at room temperature instead of 3–4 hour in the cold room
  • Flexible: purify from mammalian or bacterial cells

 

Capturem His-Tagged Purification Large Volume

  • Revolutionary solution for rapidly purifying and concentrating proteins
  • From large volumes of mammalian or bacterial cell lysate under a wide range of conditions
  • Isolate his-tagged proteins of interest with a purification system
  • Works in 10–30 min at room temperature instead of 6–10 hour in the cold room
  • High yield: up to 25 mg/unit, using a novel membrane technology instead of resin
  • Highly concentrated eluate: up to 2.0 mg/ml
  • Flexible: purify from mammalian or bacterial cells

 

Capturem His-Tagged Purification 96

  • High-throughput: Perform 96 minipreps simultaneously
  • High-capacity: Each well contains novel nylon membranes instead of resin
  • High-yield: Elute concentrated protein, up to 0.3–1 mg/ml
  • Fast and convenient: Purify proteins in just minutes, using vacuum filtration or centrifugation
  • Flexible: Purify from mammalian or bacterial cells

 

2) Cobalt columns 

TALON Single Step

  • His-Tagged Protein Purification from Bacterial Cultures
  • Complete extraction and purification in under 1 hour
  • Purify target protein directly from bacterial culture
  • Ready-to-use, prepacked columns
  • Two sizes of columns available, for large and small scale purification

 

Native vs. denaturing purification procedures using TALON resin

 

 

HisTALON Superflow Cartridges

  • Cobalt Resin FPLC Cartridges
  • Easy-to-use prepacked 1 ml and 5 ml cartridges
  • Maximize yield of biologically active protein
  • Superior purity & high yields

 

HisTALON Gravity Columns

  • Fast, Easy His-Tagged Protein Purification
  • Easy-to-use prepacked gravity columns
  • Maximize yield of biologically active protein
  • Superior purity & high yields with TALON Resin

 

TALON Spin Columns

  • Fast Small-Scale His-Tagged Protein Purification
  • Exhibits high affinity for his-tagged proteins
  • No copurification of proteins
  • Resists metal leakage
  • Performs well under a wide range of purification conditions
  • Ready-to-use spin columns
  • Each column contains 0.5 ml of resin

 

TALON Metal Affinity Resin

  • Batch or Gravity Flow Purification of His-Tagged Proteins
  • Exhibits high affinity for his-tagged proteins
  • No copurification of proteins
  • Resists metal leakage
  • Performs well under a wide range of purification conditions

 

TALON CellThru

  • His-Tagged Protein Purification from Crude Cell Lysates
  • Purification from crude cell lysate
  • Ideal for purification of membrane-bound protein or multiprotein complexes
  • No copurification of proteins
  • Low metal ion leakage
  • Fast & easy protocol

 

TALON Superflow Metal Affinity Resin

  • FPLC Purification of His-Tagged Proteins
  • Exhibits high affinity for his-tagged proteins
  • Ideal for FPLC applications
  • No copurification of proteins
  • Resists metal leakage
  • Performs well under a wide range of purification condition

 

TALON Magnetic Beads

  • His-Tagged High-Throughput Micro-Scale Purification
  • Quick and easy separation of his-tagged proteins
  • Highly selective TALON chemistry for increased purity
  • Eluted samples ideal for small volume applications

 

3) Nickel Resins

His60 Ni Resin

  • His-Tagged Recombinant Protein Purification
  • Up to 60 mg/ml of recombinant his-tagged protein
  • Low metal ion leakage in eluted his-tagged protein
  • For purification under native or denaturing conditions
  • Use for batch/gravity flow and automated FPLC purification

 

His60 Ni Magnetic Beads

  • His-Tagged High-Throughput Microscale Purification
  • Quick and easy separation of his-tagged proteins
  • Selective His60 Ni chemistry for increased purity
  • Eluted samples ideal for small volume applications

 

His60 Ni Superflow Cartridges

  • His-tagged protein purification on any LC system
  • Up to 60 mg/ml of recombinant his-tagged protein (1 ml cartridge)
  • Up to 300 mg/ml of recombinant his-tagged protein (5 ml cartridge)
  • Low metal ion leakage in eluted his-tagged protein
  • For purification under native or denaturing conditions
  • Fast and reproducible processing on any LC system, including ÄKTA

 

His60 Ni Gravity Flow Columns

  • His-Tagged Recombinant Protein Purification
  • Obtain up to 60 mg/ml of recombinant his-tagged protein
  • Low metal ion leakage in eluted his-tagged protein
  • Purify under native or denaturing conditions
  • Use for batch/gravity flow and automated FPLC purification

 

Documents and resources

For more information visit producer's website.

 

 

 

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