Inducible Protein Stabilization

Clontech

 

ProteoTuner Technology

The technology is based on a destabilizing domain that can confer its instability to a fused protein of interest. This destabilization can be rescued in a reversible and dose-dependent manner with the addition of a small molecule specific to the destabilizing domain, Shield1.

 

Destabilizing Domain

The destabilizing domain is mutant L106P of FKBP12 (12 kD), which is rapidly degraded in mammalian cells. The FKBP12 protein itself is well-characterized for its ability to form a tight complex with immunosuppressive drugs.

 

Shield1 Ligand

ProteoTuner inducible protein stabilization systems utilize ligand-dependent destabilization domains and the Shield1 ligand to reversibly stabilize and destabilize a destabilization domains-tagged protein of interest in a predictable and dose-dependent manner.

• Membrane permeable small molecule
• Binds specifically to the DD degron, to inactivate protein degradation
• No toxic effect on cells
• Can be used in cells and in animal models

 

Ligand-dependent, targeted and reversible protein stabilization

Plasmid Systems

Protein Regulation with Rapid Kinetics-ProteoTuner Systems rapidly regulate the quantity of your protein of interest, at the post translational level. The ProteoTuner systems use a powerful, unique technology to control your protein in living cells.

• Rapid kinetics: Protein level changes in minutes allows accurate functional analysis
• Precise tuning: Precise control of protein level is key to analyzing biological activity
• Reversible control: 'Protein on' to 'off' and back makes multiple studies easier

 

ProteoTuner Antibody

The Destabilization Domain Monoclonal Antibody recognizes the destabilization domain (destabilization domain-tag) in all ProteoTuner Shield Systems (N and C versions).

• Western blot and immunocytochemistry detection of destabilization domain tagged proteins
• Applicable to ProteoTuner Shield Systems (both and N and C versions)

 

Viral Systems

 ProteoTuner Lentivirus and Retrovirus Systems analyzing protein function is a key focus in cell biology research. Systems make it possible to investigate the function of a specific protein of interest directly by rapidly changing the abundance of the protein itself.

 

Documents and resources

For more information visit the producer's website.