qPCR Reagents

TaKaRa Bio

1) Preamplification

Takara PreAmp Master Mix

• 2X PCR mix optimized for unbiased preamplification of multiple (>100) targets
• Ideal for a limited (10 pg–100 ng) input of cDNA or gDNA
• Suitable for work with FFPE tissue, biofluids, laser capture microdissection or exosomes
• Amplified products can then be used for qPCR, genotyping, or target enrichment analysis
• Ready-to-use—just add cDNA or gDNA samples and primers, and then cycle 10–14 times
• Two size of Master Mix for 10 Rnxs and 40 Rxns

 

2) TB Green detection

TB Green Premix Ex Taq (Tli RNase H Plus)

• RNase H included in reaction mix: reduces inhibition from mRNA/cDNA hybrids
• Eliminates need for RNase H digestion step: when using low RNase H RTs
• Amplification of longer fragments up to 570 kb
• High sensitivity: detecs as few as 100 copies
• Excellent standard curves for various real time instruments have been established
• Compatible with all qPCR instruments
• Convenient 4 °C storage

 

TB Green Premix Ex Taq II (Tli RNase H Plus)

• Optimized for real time dye-based qPCR detection
• Easy preparation
• Extremelyhigh specificity: optimized buffer and anti-Taq antibody mediated HS polymerase
• Minimization of PCR inhibition: due to residual mRNA by premixed Tli RNAse H
• Compatible with all qPCR instruments
• Convenient 4 °C storage

 

3) Probe detection

Premix Ex Taq (Probe qPCR)

• Rapid, accurate and quantitative gene expression analysis
• For  real time PCR and TaqMan probe detection
• 2x Premix: makes pipetting easy
• Excellent amplification efficiency  and highly sensitive detection
• Contains optimized buffer and Ex Taq HS polymerase
• Includes heat-resistant Tli RNase H
• Tli RNase H minimizes PCR inhibition by residual mRNA in reactions using a cDNA template
• Compatible with all qPCR instruments: ROX reference dye included
• Convenient 4 °C storage

 

Premix Ex Taq (Perfect Real Time)

• High specificity: includes optimized buffer and Ex Taq HS for multiplex
• High sensitivity: detecs as few as 10 target copies
• Wide dynamic range: > 6 log
• Accurate quantitation: produces excellent standard curves with numerous real time instruments
• Compatible with all qPCR instruments: ROX reference dye included
• Convenient 4 °C storage

 

Documents and resources

For more information visit producer's website.

Takara PreAmp Master Mix

Real-time qPCR with TB Green detection

Real-time qPCR with probe detection