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Home > NGS Library Construction > Sequencing Accessories > Reference Standards
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Reference Standards

Horizon Discovery

The leading innovator of cell line-derived reference material

Reference Standards
Horizon Discovery

1) Next generation sequencing

The range of steps involved in NGS workflows, combined with the sheer number of biomarkers being screened, has the potential to create unprecedented levels of variability. In order to generate results with confidence, it is important to manage these potential sources of variability, from sample extraction and sample selection through to sequencing and variant calling.

Horizon's cell line-derived Reference Standards enable you to:

  • Optimize and validaten new and existing NGS platforms and assays
  • Analyze of the sensitivity and specificity of your assay
  • Gain certainty of the limit of detection and limit of quantification

 

You can choose from this products:

  • 5 Fusion Multiplex RNA Negative Control
  • Ashkenazim PGP Father Reference Standard
  • BRCA Germline II Reference Standard gDNA
  • EGFR Multiplex cfDNA Reference Standard Set
  • And a lot of other products

 

2) Sanger sequencing and qPCR

To identify and control variability (problem with DNA extraction or issues with DNA quantification), Horizon's cell line-derived Reference Standards are developed to enable you to:

  • Optimize and validate new and existing assays
  • Routinely monitor their performance
  • Analyze the sensitivity and specificity of your assay
  • Gain certainty of the limit of detection and limit of quantification

 

You can choose from this products:

  • AKT1 Wild Type Reference Standard
  • BRAF V600E Reference Standard
  • EGFR G719A Reference Standard
  • EML4-ALK Reference Standard
  • And a lot of other products

 

3) Liquid biopsy

One of the greatest challenges in liquid biopsy assays, is limit of detection. Horizon has developed a range of cell-free DNA Reference Standards, derived from cell lines, to closely mimic patient samples. With low allelic frequencies, down to 0.1%, these Reference Standards are ideal to optimize and validate your liquid biopsy assays.

 

You can choose form this products:

  • EGFR Multiplex cfDNA Reference Standard Set
  • Multiplex I cfDNA Reference Standard Set
  • Multiplex I cfDNA Reference Standard Set in Synthetic Matrix
  • Multiplex I cfDNA Reference Standard Set in Synthetic Plasma
  • Structural Multiplex cfDNA Reference Standard

 

4) Custom Reference Standards

Horizon Discovery has a broad range of cell lines available and the capability to introduce almost any mutation if not already available.

There are two ways Horizon Discovery can manufacture your custom Reference Standard:

  • Made-to-Order (MTO)
  • Fully Customized

 

Documents and resources

For more information visit producer's website. 

Next generation sequencing Reference Standards

Sanger sequencing and qPCR Reference Standards

Liquid Biopsy Reference Standards

Custom Design tool 

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