Leveraging proprietary droplet microfluidics, the platform unlocks access to DNA at the single-cell level with a novel two-step protease workflow. This methodology provides flexibility for additional applications and customization capability. DNA is ready for library preparation and NGS application.
Main features of platform
Step 1: Tapestri Instrument partitions cells into droplets along with a protease enzyme mix. Cell lysis and protease digestion then follow off-instrument enabling access to DNA for downstream amplification.
Step 2: The cell lysate is then combined with barcoded beads, primers, and reagents. Specific regions of interest are then amplified while tagging each amplicon with a unique cell barcode, preserving the cell’s identity and its mutational profile.
Next step is library preparation and sequencing on Illumina NGS platforms (MiSeq, NextSeq 550, HiSeq 2500, HiSeq 4000, NovaSeq 6000). Data are analysed and visualised by Mission Bio Software.
Tapestri Single-Cell DNA & Protein Panels
Panels can be applied across a wide number of applications, including hematologic malignancy, solid tumor, genome editing, biomarker discovery and cell and gene therapy. Panels are available as pre-designed panels or custom panels. Oligo-tagged protein antibodies can be integrated to enable concurrent measurement of proteins.
1) Catalogue panels
Featured Hematology Single-Cell DNA panels:
Tapestri Single-Cell DNA Acute Myeloid Leukemia Panel
Tapestri Single-Cell DNA Myeloid Panel
Tapestri Single-Cell DNA Chronic Lymphocytic Leukemia Panel
Additional Hematology Panels:
Hematology Protein Panels:
TotalSeq™-D Heme Oncology Cocktail (BioLegend)
Featured Solid Tumor DNA panel:
Tapestri Single-Cell Tumor Hotspot Panel
Additional Solid Tumor Panels:
2) Custom Panels
Documents and resources
For more information visit producer's website.
General purpose library preparation for Illumina NGS platforms
Unparalleled single-cell analysis including isolation and selection of cells and sample throughput
High quality analytic system with more than 25 years experience in PCR. The best choise for your PCR experiments
Best-in-class tools for sequencing library preparation from single cells and ultra-low-inputs of RNA