Tapestri Platform

Mission Bio

Leveraging proprietary droplet microfluidics, the platform unlocks access to DNA at the single-cell level with a novel two-step protease workflow. This methodology provides flexibility for additional applications and customization capability. DNA is ready for library preparation and NGS application.

 

Main features of platform

• Targeted detecting of SNVs, CNVs and proteins simultaneously from the same cell
• Obtaing information about genotype and phenotype in one step
• Single-cell DNA throughput up to 10,000 cells
• Detect rare subclones down to 0.1%
• Resolve clonal architecture
• Identify mutation co-occurrence
• Simple workflow
• User-friendly bioinformatics software
• Customizable content
• Small footprint of one square foot
• Uses novel two-step protease workflow
• Provides high sensitivity to reveal true heterogeneity of cell population
• Tool for detection of treatment response, resistance and relapse
• Compatible with Illumina NGS platforms

 

Tapestri workflow

 

Step 1: Tapestri Instrument partitions cells into droplets along with a protease enzyme mix. Cell lysis and protease digestion then follow off-instrument enabling access to DNA for downstream amplification.

Step 2: The cell lysate is then combined with barcoded beads, primers, and reagents. Specific regions of interest are then amplified while tagging each amplicon with a unique cell barcode, preserving the cell’s identity and its mutational profile.

Next step is library preparation and sequencing on Illumina NGS platforms (MiSeq, NextSeq 550, HiSeq 2500, HiSeq 4000, NovaSeq 6000). Data are analysed and visualised by Mission Bio Software.

 

Video

 

Tapestri Single-Cell DNA & Protein Panels

Panels can be applied across a wide number of applications, including hematologic malignancy, solid tumor, genome editing, biomarker discovery and cell and gene therapy. Panels are available as pre-designed panels or custom panels. Oligo-tagged protein antibodies can be integrated to enable concurrent measurement of proteins.

 

1) Catalogue panels

​Featured Hematology Single-Cell DNA panels:

Tapestri Single-Cell DNA Acute Myeloid Leukemia Panel

• Covers some of the most commonly mutated genes associate with AML
• Targets 20 genes with 127 amplicons for single-cell sequencing​

 

Tapestri Single-Cell DNA Myeloid Panel

• Targets SNVs and indels across 45 driver genes with 312 amplicons for relevant mutations
• Cover a comprehensive set of myeloid disorders including AML, MDS, CML etc. 

 

Tapestri Single-Cell DNA Chronic Lymphocytic Leukemia Panel

• Covers some of the most commonly mutated genes associate with CLL
• Targets 32 genes with 274 amplicons for single-cell sequencing

 

Additional Hematology Panels:

• Acute lymphoblastic leukemia
• Chronic myeloid leukemia
• Classic Hodgkin's lymphoma
• Diffuse large B-cell lymphoma
• Follicular lymphoma
• Mantle cell lymphoma
• Multiple myeloma
• Myelodysplastic syndromes
• Myeloproliferative neoplasms
• T-cell lymphoma (all types)

 

Hematology Protein Panels:

TotalSeq™-D Heme Oncology Cocktail (BioLegend) 

• Designed to react with 45 unique cell surface antigens
• Includies principal lineage antigens and 3 isotype control antibodiesfor characterization of cancer cells
• Provided at optimized concentrations for a ready-to-use solution

 

Featured Solid Tumor DNA panel:

Tapestri Single-Cell Tumor Hotspot Panel

• Targets SNV and indel mutation across 59 oncogenes and tumor suppressor genes
• Genes are relevant in a range of different solid tumors, e.g. breast, lung, prostate, and colorectal 

 

Additional Solid Tumor Panels:

• Breast invasive carcinoma 
• Colon adenocarcinoma
• Glioblastoma multiforme
• Kidney renal clear cell carcinoma
• Liver hepatocellular carcinoma
• Lung adenocarcinoma
• Lung squamous cell carcinoma
• Ovarian serous cystadenocarcinoma
• Pancreatic adenocarcinoma
• Prostate adenocarcinoma
• Skin cutaneous melanoma

 

2) Custom Panels

• Tapestri Designer for Custom Single-Cell DNA and Protein Panels
• Maximum flexibility to study variants relevant to your research
• Targeting of whole genes, transcripts, chromosome regions, SNPs and small indels
• Multiplex from 20 to 1000 target amplicons in a single panel
• Up to 30 oligo-conjugated antibodies to cover surface protein expression
• Simple and intuitive online design website
• Customization of existing catalog panels with targets of interest
• Expert bioinformatic and technical support for panel design inquiries

 

Documents and resources

For more information visit producer's website.

Tapestri Platform Brochure

Tapestri Single-Cell DNA & Protein Panels

Tapestri Single-Cell Custom Panels