BlotCycler - Automated Western Blotting
BlotCycler Touch is designed to automate the immunodetection step of the procedure that is traditionally performed manually.
- All steps after protein transfer to a membrane are fully automated
- The product has a total of six trays with three trays on each side
- Processing of up to 12 membranes simultaneously with two different protocols
- Simultaneous processing of 6 different Primary and 6 different Secondary antibodies
- You can program the system by choosing your blocking time, primary antibody incubation time, secondary incubation time, and the number of washing cycles
The protocol involves
- Multiple solution changes and consists of blocking
- Incubation with primary and secondary antibodies
- Washing steps
- Patented fluidic control system combined with an automated shaking motion
- Consistent and reproducible dispensing, mixing, and distribution of the reagents throughout the process
- Automation increases productivity and generates blots with higher signal and lower background
- There’s no change in workflow. You use your existing protocols and reagents.
Precision Biosystems offers reagents
CY-BLUE: Precision Blue Film
- Optimized for use in all blue and green light chemiluminescens systems
- Coated on both sides for improved consistency and overall performance
PrecastGel
- Polyacrylamide minigels for SDS-PAGE
- Reproducible protein separation for the analysis of protein mixtures and downstream Western blotting
- For better separation gels use Tris-glycine Laemmli electrophoresis buffers
- Gel cassettes have labeled wells for easy loading
- Compatible with multiple vertical tank electrophoresis systems
Precision Stripping Buffer
- Optimized to efficiently remove primary and secondary antibodies but leave antigen attached to the membrane
- Saves your primary antibodies.
- After running a protocol, use Precision Stripping Buffer, and you can reuse your membrane up to 10 times!
Documents and resources
For more information visit producer's website.
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