Targeted RNA-Seq
Clontech
Tool for studies involving a predetermined set of genes
Targeted RNA-Seq allows the analysis of transcripts of interest including those resulting from alternative splicing or gene fusion events and uses fewer sequencing reads than would be required in the context of global analysis
SMARTer Target RNA Capture for Illumina
- Employs biotinylated DNA probes that are designed by the user
- Probes hybridize with transcripts of interest an bound by streptavidin-coated capture beads
- Inputs of 10 ng - 1µg of total RNA
- User-designed DNA probes enrich for transcripts of interest
- Ligation-free protocol
- Recommended for analysis of up to 100 genes per reaction
- cDNA synthesis is performed on hybridized transcripts using SMART-Seq v4 chemistry with oligo(dT) priming
Documents and resources
For more information visit producer's website.
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