Genome engineering products

Dharmacon

Optimized tools and service for high-confidence genome engineering

1) CRISPR-Cas9 Gene Editing

The Dharmacon Edit-R CRISPR-Cas9 platform greatly simplifies the workflow of permanently knocking out genes. The approach includes guaranteed, predesigned, ready-to-use guide RNAs and enables fast assessment of multiple target sites per gene for multiple genes.

You can choose from these products:

CRISPR Guide RNA - ready-to-use lentiviral and synthetic reagents to guide Cas9 cleavage
Cas9 Nuclease
CRISPR Controls and Detection Primers
CRISPR-Cas9 Screening Libraries - pooled sgRNA or arrayed crRNA for high-throughput studies
HDR Donor Templates - synthetic single-strand DNA oligonucleotide donors for precise genomic modification with the homology-directed repair (HDR) pathway

2) CRISPRa for Transcriptional Activation

Reagents provide a efficient set of tools to study a gene’s function by overexpression in its native context. Predesigned reagents for human and mouse genes make it easy! Using a deactivated or dead Cas9 nuclease fused to transcription activators, like dCas9-VPR, in combination with a guide RNA in either a synthetic or lentiviral format, the endogenous expression of a gene can be up-regulated.

Dharmacon offers these products:

CRISPRa Guide RNA
Edit-R dCas9-VPR
CRISPRa Controls
CRISPRa Screening Libraries

3) RNA Interference

RNAi products contain the most complete portfolio of innovative tools for transient, long-term, inducible and in vivo RNAi applications. RNAi reagents are complemented with a complete suite of products for delivery, control and confirmation of results.

You can choose from these products:

siRNA - pre-designed genome-wide reagents for siGENOME, ON-TARGETplus, and Accell product lines for human, mouse, and rat
shRNA - SMARTvector, GIPZ, TRIPZ and TRC lentiviral shRNA products designed for optimal gene silencing
microRNA - best-in-class microRNA modulation tools including miRIDIAN microRNA Mimics, microRNA Hairpin Inhibitors and shMIMIC Lentiviral microRNAs
RNAi Screening Libraries- the widest selection of RNAi screening collections available. Choose from whole genome, gene families, or custom libraries.
RNAi Controls - negative and positive controls as well as transfection indicators for all product lines.
Ancillary Reagents - all necessary ancillary reagents, including buffers, water, and specialized cell culture media

4) cDNAs and ORFs

These products can be used to overexpress particular genes of interest. Choose from individual clones, lentiviral ORFs, and genome-scale libraries.

You can choose from these products:

Mammalian cDNAs
Mammalian ORFs
Non-mammalian cDNAs & ORFs
cDNA and ORF Libraries

5) Transfection

Transfection reagents for a wide range of applications. Lipid-based transfection reagents provides high efficiency delivery, low cellular toxicity and broad dynamic range.

You can choose from these products:

DharmaFECT for small RNA transfection
DharmaFECT Duo for co-transfection of plasmid and small RNA
DharmaFECT kb for plasmid DNA transfection
Accell siRNA - self delivering siRNA -for difficult to transfect cell types

6) Viral Packaging

Package 2nd or 3rd generation viral vectors to consistently generate high-titer viral particles for gene silencing or gene expression studies.

Dharmacon offers Trans-Lentiviral shRNA Packaging Kits:

Trans-Lentiviral shRNA Packaging System - package your shRNA viral vector constructs into high-titer lentiviral particles for efficient transduction and gene silencing in the cell or tissue type of interest
Trans-Lentiviral ORF Packaging System - package your ORF viral vector constructs into high-titer lentiviral particles for efficient transduction and gene expression in the cell or tissue type of interest

7) Services

Online design tools and extensive synthesis options to create custom products specific to your application.