Viral delivery of sgRNA/Cas9

TaKaRa Bio

AAVpro CRISPR/Cas9 Helper Free System is the adeno-associated viral system suitable for hard to transfect cells. System uses the two vectors, because the AAV genome is quite small, so Cas9 is split into the two vectors. Then the vectors are cotransducted into the target cell and the result is expression of whole complex of Cas9 and targeted sgRNA. 

 

 

Lenti-X CRISPR/Cas9 System also contains two types of plasmids – one lentiviral plasmid with Cas9 and the second prelinearized sgRNA plasmid. Stable expression of Cas9 helps to reduce assay variability when screening sgRNA libraries. 

 

 

Lenti-X Tet-On 3G CRISPR/Cas9 System is based on Tet-on 3G technology to achieve doxycycline-inducible Cas9 expression and control Cas9 expression with very low background. 

 

Documents and resources

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