Pluripotency and Differentiation

TaKaRa Bio

Cellartis iPS Cell to Hepatocyte Differentiation System

• Hepatocytes derived from human iPS cells
• Can be alternatively used instead of primary hepatocytes
• They have sufficient expression levels of enzymes, transporters and are stabile functional in the culture
• Combines a standardized protocol with optimized, ready to use media, supplements and coating reagents
• Provides a complete solution for directed differentiation of disease specific or patient specific hiPS cells into hepatocytes

 

There are the various tools how to check all the parameters of the stem cell culture

iPS Efficiency Check qPCR Kit

• ​​For monitoring quantitate expression levels of key senescence markers
• Contains qPCR primer mixes specific for key senescence markers
• Complete kit to verify iPS generation
• Includes RNA extraction, reverse transcription and qPCR reagents

 

Pluripotency Check PCR Kit

• For insure, that the mouse stem or iPS cells are indeed pluripotent
• Contains 16 prevalidated PCR primer mixes
• Confirms the iPS cells by differentiating between endogenous and exogenous gene expression

 

Primer Arrays

• For monitoring of the differentiation of the cells by qPCR Primer mixes
• PrimerArray Hepatic Differentiation
• Suitable for the analysis of the hepatocyte differentiation
• PrimerArray Embryonic Stem Cell Array
• Suitable for the expression analysis to screen pluripotency of embryonic stem cell and iPS cells
• Human Mitochondrial DNA Monitoring Primer Set
• Designed to quantify the relative number of copies of human mitochondrial DNA (mtDNA)
• Using nuclear DNA (nDNA) content as a standard by real-time PCR

 

Takara offers also the Differentiation Kits from the Cellartis line

Cellartis Hepatocyte Differentiation Kit

• Generates highly homogenous hepatocyte cultures starting from definitive endoderm cells
• Tested in more than 25 different stem cell lines

 

Cellartis Definitive Endoderm Differentiation Kit

• Contains a complete media set and ready to use coating
• For the differentiation of human iPS cells into definitive endoderm cells in monolayer culture
• The kit includes the Cellartis DEF-CS 100 Culture System
• Generates more than 80 % of the SOX17 positive cells

 

Documents and resources

For more information visit producer's website.