Culture Systems for Stem Cells

TaKaRa Bio

1) Maintenance media as the serum free products are ideal for Embryonic Stem, Embryonic Germ and iPS cell culture.

 

Cellartis DEF-CS Culture System

• Robust culture system for efficient expansion of human iPS cells in a feeder free and defined environment
• This system eliminates the need for cell selection, because there is no detectable background differentiation
• The kit includes 500 ml of basal medium, additives and coating compound

 

Cellartis DEF-CS 500 Xeno-Free Culture Medium

• Medium without antibiotics
• iPS cells are cultured in conditions completely free from humand or animal derived components
• The cells are cultured in absence of feeder cells, the substrate iMatrix-511 is used
• This medium is available also in GMP variant

 

GS1-R Medium

• Suitable for maintenance, propagation and manipulation of rat embryonic stem and embryonic germ cells
• GS1-R uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK

 

GS2-M Medium

• Completely defined serum free cell culture media for human and mouse embryonic stem and iPS cell lines
• Uses selective small molecule inhibitors to block differentiation signals from GSK3β and ERK/MEK
• Maintains ES and iPS cells in a pluripotent state.

 

iSTEM Medium  

• Defined for mouse embryonic stem cells
• Captures mouse ES cells in their basal state by blocking the inductive pathways of differentiation with selective small molecule inhibitors

Product	Species	Cell Type	Includes Culture Matrix	Feeder Free, Defined
Cellartis DEF-CS 500 Culture System	Human	iPS	X	X
Cellartis DEF-CS 500 Xeno-Free Culture Medium	Human	iPS		X
GS1-R	Rat	ES and EG
GS2-M	Human, Mouse	ES and iPS		X
iSTEM	Mouse	ES		X

 

2) Differentiation media promote Embryonic Stem and Neural Stem cell differentiation along the neural lineage.

 

NDiff 227

• Defined, serum free, N2 and B-27
• Supplemented medium for neural differentiation of mouse embryonic stem cells in adherent monoculture

 

RHB-A

• Proprietary, fully defined and serum free medium
• For isolation of pure populations of adherent human and mouse neural stem cells

 

RHB-Basal

• Animal component free and contains no neuronal supplements, the medium can be customized

Product	Species	Cell Type	Details
NDiff 227	Mouse	ES	Neural differentiation contains N2 and B-27
RHB-A	Human, Mouse	NS	Propagation and cell-type specific differentiation contains growth factors
RHB-Basal	Human, Mouse	NS	Basal formulation of RHB-A does not contain growth factors or neuronal supplements

 

3) Supplements from the NDiff product line allows the derivation, maintenance and expansion of embryonic stem and neural stem cells as well as the differentiation into the neural lineage.

 

NDiff N2

• Completely defined serum free cell culture
• Supplement for the derivation, maintenance, expansion or differentiation
• For human and mouse stem cells and tissue derived neural stem cells

 

NDiff N2-AF

• Animal free version of NDiff N2 what contains no components of animal origin

Species	Cell Type	Serum Free, Defined	Animal free	Product
Human, Mouse	ES and NS to Neural lineage	X		NDiff N2
		X	X	NDiff N2-AF

 

 

Documents and resources

For more information visit producer's website.