Protein Detection

TaKaRa Bio

CLEARLY Protein Ladder Molecular Weight Markers

• Convenient and ready-to-use Ladder sets
• Mixture of 11 highly purified recombinant proteins
• Load directly on gels - no pre-heating is necessary
• CLEARLY Protein Ladder (Unstained) is recommended as a precise molecular weight standard.
• Easily monitor the efficiency of protein transfer to a western blot

 

TaKaRa CBB Protein Safe Stain

• Highly sensitive Coomassie Brilliant Blue G-250-based stain detects as little as 8 ng of protein
• Visualize protein bands in approximately 5 min, with maximum sensitivity after 60 min
• Safe handling—does not contain methanol or acetic acid; destain gels with deionized water
• This product can be used to stain SDS-PAGE or native PAGE gels quickly

 

Western Blot Blocking Buffer

Suppresses non-specific signals is critical to obtaining clear, specific Western blot results. TaKaRa Bio offers several specialized, ready-to-use blocking buffers.

1) Western BLoT Blocking Buffer (Protein Free)

• completely chemically defined and free of protein components 
• Provides higher sensitivity than other commonly used blocking agents (nonfat milk)
• Excellent for detection of phosphorylated proteins

2) The Western BLoT Blocking Buffer (Fish Gelatin)

• Blocking reagent of fish origin
• It is less likely to cross-react with mammalian antibodies than conventional blocking reagents, such as nonfat milk or BSA

 

Western BLoT Chemiluminescent Substrate Series 

Chemiluminescent substrates for detecting horseradish peroxidase (HRP)-labeled antibodies used during Western blotting.

Features

Product name	Features	Detection Limit	Detection Method	Membrane
Western BLoT Chemiluminescence HRP Substrate	Stronger signal than other equivalent ECL substrates.	Picogram	X-ray film	Nitrocellulose, PVDF
Western BLoT Quant HRP Substrate	Long-lasting, stable signal. High sensitivity and quantitative accuracy.	Low picogram	CCD camera, X-ray film	Nitrocellulose, PVDF
Western BLoT Hyper HRP Substrate	Quantitative detection even for low-abundance proteins. Strong signal when high dilution factor for the primary antibody is used.	Femtogram	CCD camera, X-ray film	Nitrocellulose, PVDF
Western BLoT Ultra Sensitive HRP Substrate	Highest sensitivity in the series.	Low femtogram	CCD camera, X-ray film	Nitrocellulose, PVD

Western BLoT Immuno Booster

• Highly sensitive detection of low abundance proteins without having to change HRP substrate or the amount of antibody
• Protein-free version is available
• For variety of immunoassays, including Western blotting and ELISA
• Enhance antigen-antibody interactions and thereby increase final detection sensitivity

 

Western BLoT Rapid Detect v2.0

• Rapid detection of primary antibodies using an antibody-free IgG Detector Solution (HRP-labeled).
• Simultaneous detection of multiple primary antibodies
• Detection of minute quantities of antigen that cannot be detected using conventional techniques with a labeled secondary antibody

 

Western Blot Stripping Buffer

• Efficient antibody removal from Western blot membranes with little loss of bound protein
• Fast, simple protocol (immerse blot in Stripping Buffer and incubate at room temperature for 30 minutes)

 

Documents and resources

For more information visit producer's website.