Two-Step RT-qPCR

TaKaRa Bio

1)  PrimeScript RT Master Mix

• Master mix for reverse transcription prior qPCR
• Fast cDNA synthesis: ready for qPCR in 15 minutes
• Uses PrimeScript RTase to reverse-transcribe any RNA, even RNA with strong secondary structure
• This kit is best suited for two-step RT-qPCR
• Random 6 mers and Oligo dT primer in the premix for full RNA coverage
• Simple to use and suitable  for high throughput: Master Mix reduces pipetting steps and contamination risks

 

2) PrimeScript RT Reagent Kit

• Separate components for reverse transcription prior qPCR

• Fast cDNA synthesis: ready for qPCR in 15 minutes
• Includes random 6 mers and oligo dT primer for use as reverse transcription  primers
• Uses PrimeScript RTase to reverse-transcribe any RNA, even RNA with strong secondary structure
• This kit is best suited for two-step RT-qPCR

 

3) PrimeScript RT Reagent Kit with gDNA Eraser

• Accurate gene expression analysis 
• Eliminates genomic DNA in jist 2 minutes, avoids gDNA quantification
• First-strand cDNA synthesis to be used for real-time PCR
• Fast: reverse transcription is complete in just 15 minutes
• Random 6 mers and Oligo dT primer in the Primer Mix for full RNA coverage

 

 

Documents and resources

For more information visit producer's website.