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Chemiluminescent Reporters

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Home > Protein Analysis > Fluorescent Proteins and Reporters > Chemiluminescent Reporters
Fluorescent Proteins and Reporters
  • Fluorescent Protein Antibodies
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Chemiluminescent Reporters

TaKaRa Bio

Detecting and measuring the expression of any recombinant protein and gene activity

Chemiluminescent Reporters
TaKaRa Bio

Beta-Galactosidase Assay and LacZ Vectors

The beta-galactosidase assay is commonly used as a reporter, to monitor transfection efficiency, and for promoter/enhancer studies.

Colorimetric assay

  • The Beta-Galactosidase Staining Kit
  • Complete system for colorimetric detection of beta-galactosidase expression in mammalian cells

Chemiluminescent assay

  •  The Luminescent Beta-Galactosidase Reporter System 3
  • Provide quantitative measurement of beta-gal activity using a chemiluminescent substrate

 

Flow chart of chemiluminescent beta-galactosidase assay

 

pBeta-Gal-Basic

  • Eukaryotic promoter and enhancer sequences
  • Serve as a negative control or to clone promoters for characterization

 

pBeta-Gal-Control

  • Contains the SV40 promoter and enhancer sequences
  • Can be used as a positive control or as a reference for comparing the activities of promoters and enhancers

 

pCMV-LacZ

  • Constitutive mammalian reporter vector
  • Can be used as a reference or control plasmid
  • Expresses extremely high levels of beta-galactosidase from the human cytomegalovirus immediate early promoter (PCMV IE) 

 

Luciferase assay

In Vivo Luciferase Imaging Kit

  • Provides a bioluminescent assay
  • Simplifies the analysis of promoter function in live cells or live animals
  • Fast and easy—detect promoter activity in live cells and live animals
  • For live-cell and live-animal imaging
  • Allows repeated assaying over time

 

Ready-To-Glow Secreted Luciferase Assay

  • Based on the secreted Metridia luciferase reporter.
  • Combines the advantages of a live-cell assay with the sensitivity of an enzyme-based system
  • Secreted luciferase assay protocol eliminates cell lysis
  • 2–4 fold higher signal than firefly or Renilla luciferase
  • Single transfection allows multiple time points from same cell

 

ProLabel

ProLabel Chemiluminescent Assay

  • Provide sensitive, chemiluminescence-based assays
  • For detecting and measuring the expression of any recombinant protein of interest
  • Western blot kit alternative: highly sensitive, easy to use
  • A quantitative enzymatic assay that’s simple and fast
  • High-throughput compatible

 

Secreted Alkaline Phosphatase

Secreted Alkaline Phosphatase Assay

  • The Great EscAPe SEAP 2.0 System uses a secreted alkaline phosphatase assay
  • For analysis cis-acting DNA sequences and trans-acting factors
  • One step assay, for monitoring promoter activity
  • 10-fold higher sensitivity then firefly luciferase
  • No-cell-lysis protocol

 

Documents and resources

For more information visit producer's website.

 

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