HTG EdgeSeq System

HTG Molecular Diagnostics

• Adapted chemistry and automate platform simplify NGS library preparation workflow
• Reliable, reproducible analysis of hundreds or thousands of biomarkers
• Suitable for variety of samples with very low input requirements
• 15 μl of biofluids, one 5 μm FFPE section, 10 ng of extracted RNA, ≥ 3.000 cells, 500 µl of PAXgene
• Provides excellent performance on FFPE samples
• Simple, extraction-free sample preparation
• The pooled libraries may be sequenced using most NGS platforms
• Automated workflow with sample-to-result turnaround times in 2 days with minimal hands on time
• Simplified data output

 

HTG EdgeSeq workflow 

Extraction-free chemistry significantly reduces sample input requirements, preserves valuable samples and eliminates time consuming and technically challenging steps like cDNA synthesis, size selection, removal of rRNA, mRNA end repair, and adapter ligation resulting.

 

 

 

HTG EdgeSeq Assays:

HTG offers a variety of profiling assays for research use (RUO):

 

HTG Transcriptome Panel

• Simultaneously interrogates 19,398 targets 
• Designed to provide extensive coverage of most human mRNA transcripts including isoforms
• Data for up to 96 samples are generated in less than three days
• Material: Normal or neoplastic FFPE sections, extracted RNA, PAXgene

 

HTG EdgeSeq miRNA Whole Transcriptome Assay

• Simultaneous, quantitative detection of 2.083 human miRNA transcripts
• Material: plasma or serum, FFPE section, extracted RNA from frozen or FFPE, PAXgene, cells

 

HTG EdgeSeq Oncology Biomarker Panel

• Simultaneous, quantitative detection of 2,549 transcripts associated with tumor biology
• Helps to identify therapeutic targets and drug response markers
• Material: FFPE section, extracted RNA from frozen and FFPE, PAXgene, cells

 

HTG EdgeSeq Precision Immuno-Oncology Panel

• Analysis of 1,392 genes focused on tumor/immune interaction
• Provides broader view of the immune response in solid and liquid tumors
• Designed to measure the immune response both inside the tumor and the surrounding microenvironment
• Tumor subtyping based on defined molecular expression and immune response phenotypes 
• Material: FFPE section, extracted RNA, PAXgene

 

HTG EdgeSeq Immune Response Panel

• Measures 2,002 immune response mRNA targets in a single RNA extraction-free panel
• Profiling of genes implicated in the immune response (pathogens, variety of autoimmune diseases) 
• Material: One 5 µm section, 500 µl of PAXgene

 

HTG EdgeSeq Pan B-Cell Lymphoma Assay

• Measure 298 transcripts in a single well
• Broad profile of genes associated with B-cell lymphomas
• Addresses the heterogeneity of B-Cell lymphomas, including published signatures and drug targets
• Material: one 5 µm FFPE section

 

HTG EdgeSeq Mouse mRNA Tumor Response Panel

• Measurement of  1,659 mouse mRNA targets in a single well
• Broad profile of genes associated with oncology, immunity, and inflammation
• For modeling oncogenesis, investigational therapeutic response studies
• For studying the immune response to mouse and patient-derived xenografts 
• Material: FFPE section, extracted RNA from frozen or FFPE, cells

 

HTG EdgeSeq DLBCL Cell of Origin Assay

• Simultaneous, quantitative detection of 92 transcripts associated with B-cell lymphomas
• Characterization into activated B-cell like, germinal center B-cell like or unclassified subtype
• Material: one 5 µm FFPE section 

 

 

 

Services

VERI/O laboratory services

• Offers assay development and sample processing services of molecular profiling 
• Ideal for retrospective studies
• For biopharma clients and academic collaborators in translational research

 

HTG Qualified Service Provider program

• Global network of laboratories who can provide to run HTG assays for customers' needs 
• Retrospective molecular profiling and prospective clinical trials

 

Documents and resources

For more information visit producer's website

HTG EdgeSeq System Workflow Brochure

HTG Transcriptome Panel Gene List

HTG EdgeSeq miRNA Whole Transcriptome Assay Cross-Species Gene List

HTG EdgeSeq Oncology Biomarker Panel Gene List

HTG EdgeSeq Precision Immuno-Oncology Panel Gene List

HTG EdgeSeq Immune Response Panel Gene List

HTG EdgeSeq Pan B-Cell Lymphoma Panel Product Sheet

HTG EdgeSeq Mouse mRNA Tumor Response Panel Gene List

HTG EdgeSeq DLBCL Cells of Origin Assay Gene List