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Single Cell and Ultra-Low-Input RNA-Seq

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Home > NGS Library Construction > Whole Transcriptome Analysis > Single Cell and Ultra-Low-Input RNA-Seq
Whole Transcriptome Analysis
  • Single Cell and Ultra-Low-Input RNA-Seq
  • Asteria Single-cell RNA-seq Benchtop Kit
  • Total RNA-Seq
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Single Cell and Ultra-Low-Input RNA-Seq

Clontech

Best-in-class tools for sequencing library preparation from single cells and ultra-low-inputs of RNA

Single Cell and Ultra-Low-Input RNA-Seq
Clontech

Robust and sensitive enough NGS library preparation methods for reproducibly capture RNA molecules present in minute quantities.

 

cDNA SYNTHESIS FROM SINGLE CELL

SMART-seq Single Cell Kit

  • Uses robust chemistry for single-cell RNA analysis with ultimate sensitivity
  • Generates high-quality, full-length cDNA directly from different single cell types
  • It has been validated with 2 pg of total RNA input
  • Good results with low RNA content cells like peripheral blood mononuclear cells
  • cDNA is compatible with lllumina platform-specific library preparation kits
  • Easy, plate-based workflow - direct input of single cells isolated by FACS or other methods
  • Highly scalable workflow - high-throughput capabilities and automation-friendly
  • Compatible with droplet-based technologies, such as 10X Genomics

 

cDNA SYNTHESIS FROM ULTRA-LOW-INPUT RNA

  • cDNA synthesis from 1 - 1,000 cells, or 10 pg –10 ng high-quality (RIN>8) total RNA
  • Kits use oligo(dT) priming
  • Rely on proprietary SMART (Switching Mechanism at 5’ end of RNA Template) technology
  • SMART technology ensures full-length unbiased mRNA coverage
  • Powerful approach for resolving biologically relevant differences between individual cells
  • Analysis of rare or precious samples (stem cells, circulating tumor cells and tissue biopsies)
  • Improved chemistry relative to previous versions and locked nucleic acids (LNA)-enhanced oligos
  • Accurate representation of GC-rich transcripts
  • Compatible with Illumina or Ion Torrent sequencing platforms

 

You can choose from available kits

1) Full-length cDNA

SMART-Seq HT Kit

  • for high-throughput applications
  • cDNA synthesis from 1 - 100 cells or 10 pg –1 ng total RNA

 

SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing

  • cDNA synthesis from 1 - 1,000 cells or 10 pg –10 ng total RNA

 

SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System

  • cDNA synthesis from single cells

 

2) 3'​ end only

SMART-Seq v4 3'​ DE Kit

  • cDNA synthesis from 1 - 100 cells or 10 pg –1 ng total RNA
  • Compatible  with Illumina library prep only

 

 

LIBRARY PREPARATION

SMART-Seq Stranded Kit

  • Illumina ready library generation from 1-1000 cells or 10 pg-10 ng of total RNA
  • For RNA of any integrity (RIN 2-10)
  • Streamlined workflow: 7 hours from sample to sequencer
  • Unparalleled sensitivity for single-cell, full-length total RNA sequencing 
  • Retains strand of origin information
  • Incorporated rRNA depletion step using ZapR and R-Probes optimized for single-cell
  • Uses a random-priming approach to capture the entire transcriptome
  • Product is available in 3 sizes of 12 rxns, 48 rxns and 96 rxns

 

 

Documents and resources

For more information about SMART-seq Single Cell Kit visit producer's website.

For more information about cDNA synthesis visit producer's website.

For more information about SMART-Seq Stranded Kit visit producer's website.

SELECTION GUIDE Next-Gen Sequencing Kits 

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