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SMARTer ICELL8 Single-Cell Analysis Systems

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SMARTer ICELL8 Single-Cell Analysis Systems

TaKaRa Bio

Unparalleled single-cell analysis including isolation and selection of cells and sample throughput

SMARTer ICELL8 Single-Cell Analysis Systems
TaKaRa Bio

Takara Bio offers:

1) SMARTer ICELL8 cx Single-Cell System

- compact version of the original ICELL8 for single cell sequencing automation

2) SMARTer ICELL8 Single-Cell System

- original system, consists of several separate parts

 

ICELL8 cx and ICELL8 Systems Features:

  • Evaluation of up to 8 sample types or conditions on each chip
  • Unbiased isolation of 1,000–1,800 single cells in a 5,184-nanowell chip
  • Maximizing of throughput with rapid dispensing and imaging
  • Analysis of cells with sizes of 5-100µm with unbiased cell isolation
  • Isolation of various cells (e.g. nuclei, cardiomyocytes, B lymphocytes, neurons, spheroids, etc.)
  • Imaging to differentiate single from multiple cells and perform live/dead analysis with fluorescence
  • Multiple imaging channels (red, blue, green)
  • Choice of specific cells to process using advanced imaging software
  • Maximising experimental efficiency by analysing only cells of interest
  • Reagents optimised to enhance cell separation and viability
  • Blank chips and preprinted chips for specific applications
  • Flexibility to use pre-validated SMARTer NGS chemistries or developped custom applications

 

      

 

MultiSample NanoDispenser (MSND)

  • Deposits nanoliter volumes of cells and reagents into the ICELL8 Chip
  • Capable of dispensing 8 unique samples evenly across the chip in 15 minutes
  • Rapid dispensing helps to ensure cell viability throughout the cell isolation process
  • Temperature and humidity controlled environment

 

 

Imaging Station

  • Easy to use optical system, purpose-configured for the ICELL8 Chip
  • Rapid image capture of individual wells on the chip
  • Includes fluorescence detection of commonly used dyes in single cell research
  • Designed to interface directly with CellSelect software

 

CellSelect Software

  • Interrogates images of each well
  • Evaluates parameters to determine the number of cells present and the cell viability state
  • Identify wells with single cells and evaluate viability to recommend wells for downstream processing

 

 

Applications

  • Differential expression by 3' end counting
  • TCR profiling and 5′ differential expression
  • Full-length transcriptome analysis
  • ATAC-seq

 

1) SMARTer ICELL8 cx Single-Cell System

​Full system includes:

  • SMARTer ICELL8 cx Thermal Cycler
  • Built-in imaging capabilities
  • Integrated SMARTer ICELL8 cx CellSelect Software

 

  • Advanced, integrated automation platform
  • End-to-end automation for dispensing and isolating single cells in 5,184-nanowell chips
  • Large-bore nozzle for unbiased cell size isolation

 

2) SMARTer ICELL8 Single-Cell System

System consists of:

  • MultiSample NanoDispenser (MSND)
  • Imaging Station
  • CellSelect Software

 

 

Documents and resources

For more information visit producer's website.

SMARTer ICELL8 cx Single-Cell System Video 

SMARTer ICELL8 cx Single-Cell System User Manual 

SMARTer ICELL8 cx CellSelect Software User Manual

SMART-Seq ICELL8 cx Application Kit User Manual

ICELL8 MultiSample NanoDispenser User Manual

ICELL8 Imaging System User Manual

CellSelect Software User Manual

 

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