Tet Systems - Tet-On 3G

Clontech

The Tet-On 3G Tetracycline-Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available.

 

 

EF1-Alpha Promoter Versions of Tet-On 3G

EF-1 alpha promoter versions of the Tet-On 3G Tetracycline Inducible Expression System  provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells.

• View kit components
• Tetracycline transactivator, Tet-On 3G, is expressed from an EF1-alpha promoter
• Very low background expression
• High induced expression

 

Bidirectional Tet-On 3G Inducible Expression Systems

The response vector supplied with these Tet-On 3G tetracycline-inducible expression systems contains a bidirectional version of our premium TRE3G promoter (PTRE3G-BI) that allows for simultaneous, equivalent and inducible expression of two transgenes.

• Equivalent co-regulated and simultaneous expression of two genes
• Indirectly monitor expression of a target gene via co-induced expression of a bright green or red fluorescent protein
• Same tight control as all Tet-On 3G Systems
• Highly sensitive to doxycycline

 

Bicistronic IRES Tet-On 3G Inducible Expression Systems

The response vector supplied with these Tet-On 3G tetracycline-inducible expression systems express two transgenes from our premium TRE3G inducible promoter (PTRE3G).

• Tightly controlled inducible expression of two genes of interest from the same bicistronic transcript
• Monitor induced expression of a single gene using mCherry or ZsGreen1 fluorescent proteins
• Two transgenes or one transgene and a bright fluorescent protein expressed from a single IRES bicistronic transcript

 

Lentiviral Tet-On 3G Inducible Expression Systems

The Lenti-X Tet-On 3G Inducible Expression Systems combine the broad cellular tropism of lentiviral gene transfer technology with the power and versatility of our state-of-the-art tetracycline-regulated gene expression system.

• Low basal expression, high maximal expression, highly controllable
• Lenti-X Packaging Single Shots (VSV-G), included with each system, generates high titers with a very high safety profile
• TRE3G promoter provides very tight control of transcription

 

Tet-On 3G Lentiviral Particles—Purified and Concentrated

Ready-to-use, purified, high titer lentiviral particles that deliver the Tet-On 3G transactivator to target cells.

• Premium quality ready to use Tet-On 3G lentivirus
• Very high titer; functional titer of >1 x 109 transduction units per ml (TU/ml)
• Functional titer provided, not a just a particle count
• High purity - transduce target cells without cytotoxicity

 

Retroviral Tet-On 3G Inducible Expression Systems

The Retro-X Tet-On 3G Inducible Expression System combines the efficiency of retroviral gene transfer with the power of our premium tetracycline-regulated gene expression technology.

• Outstanding induction control and extremely low basal expression for a broad range of dividing cell types
• TRE3G promoter is optimized for very tight transcriptional control
• Supplied with our best-performing retroviral packaging system (Retro-X Universal)

 

Adeno-X Expression System 3 (Tet-On 3G Inducible)

Clontech’s Adeno-X Adenoviral System 3 (Tet-On 3G Inducible) combines the tightest and most sensitive control of gene expression with the most advanced commercially available adenoviral vector system.

• Very tight control of gene expression
• Simple-to-use, all-in-one tetracycline inducible system
• The most advanced adenoviral gene delivery technology
• Easiest adenoviral system to use; cloning is even simpler than standard plasmid cloning

 

Tet-On 3G Cell Lines for Tetracycline-Inducible Expression

Tet-On 3G cell lines greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium.

• Cell lines with high inducibility
• Rapidly create an inducible expression system for your gene of interest.
• Premade Tet-responsive cell lines reduce development time/effort by half

 

Documents and resources

For more information visit producer's website.